000			03440nam a2200301Ia 4500
003			00IHBT
005			20140908102147.0
008			130910s9999 xx 000 0 und d
020			_a0896035662
041			_hENG
082			_a579.3
100			_aEdwards, Clive [Ed.] _d1949- _97683
245			_aEnvironmental Monitoring of Bacteria _cEdited by Clive Edwards
260			_aNew Jersey _bHumana Press _c1999
300			_ax, 333p.
440			_aMethods in Biotechnology _vv. 12 _93782
500			_a Some problems posed by natural environments for monitoring microorganisms -- Sampling sediment and soil: use of coring devices -- Sampling water bodies: tangential flow filtration -- Dielectrophoresis - Flow cytometry and cell sorting: rapid analysis and separation of individual bacterial cells from natural environments -- Magnetic particle-based separation techniques for monitoring bacteria from natural environments -- DNA extraction from natural enviornments -- Automated sequencing of DNA retrieved from environmental samples -- Analysis of DNA sequences -- Fluorescent polymerase chanin reaction/restriction fragment lenght polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments -- Recovery and analysis of ribosomal RNA sequences from the environment -- Application of denaturing gradient gel electrophoresis to microbial ecology -- Reporter gene expression for monitoring microorganisms -- Characterizing microorganisms in the environment by fatty acid analysis -- Fluorescent in situ hybridization and the analysis of the single cell -- Specific detection, viability assessment, and macromolecular staining of bacteria for flow cytometry -- Confocal laser scanning microscopy of environmental samples -- Monitoring microbial activities using membrane inlet mass spectrometry -- Experimental biofilms and their applications in the study of environmental processes -- Establishment of experimental biofilms using the modified robbins device and flow cells
500			_aClive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing and specifically address the difficult problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional microbiological methods. Environmental Monitoring of Bacteria takes full advantage of the exciting research in recent years that has challenged some long-held principles of traditional microbiology and gives timely and practical access to techniques that allow the study of bacterial ecology as communities, single cells, or at the molecular level.
650			_aBacteria _94129
650			_aBiotechnology _92483
650			_aMicrobial ecology _94693
650			_aEnvironmental monitoring _97684
650			_aEnvironmental Microbiology _97685
650			_aMolecular biology _93043
856			_uhttp://www.loc.gov/catdir/enhancements/fy0825/99201906-d.html _yPublisher description
856			_uhttp://www.loc.gov/catdir/enhancements/fy0825/99201906-t.html _yTable of contents only
942			_cBOOK _2ddc
999			_c4395 _d4395
856			_uhttp://14.139.59.218:80/cgi-bin/koha/opac-detail.pl?biblionumber=4395 _yhttp://14.139.59.218:80/cgi-bin/koha/opac-detail.pl?biblionumber=4395